細胞一氧化氮測量系統，ArrowSTRAIGHT? various methods for quantitatively determining the concentration of both NO2- and NO3- ions. The most common methods involved the use of the Griess reagent which reacts with NO2- ion to produce a stable azo end product which is purple in color and can be quantified using colorimeteric or spectrophotometric analytical techniques. There are three basic problems with this colorimetric technique. First of all, the Griess reagent only reacts with the NO2- compound thereby requiring an additional difficult step to completely reduce NO3- ion to NO2-. Secondly, like with all colorimetric methods, the Griess technique can be affected by original sample color or turbidity. Thirdly, this method is very time consuming and tedious because it involved several intermediate chemical reactions.
細胞一氧化氮測量系統，ArrowSTRAIGHT?Recently a potentially more accurate, less time consuming, and less tedious method has been developed for assaying both NO2- and NO3- which relies on the use of ion selective electrodes for both NO2- and NO3- ions. These electrodes have been miniaturized to be able to measure samples down to 50 to 100 microliters with detection limits down to the low microMolar range for both species. Since both NO2- and NO3- ions can be measured individually no reaction to reduce NO3- to NO2- is required. This micro electrode system comes complete with a sophisticated ion analyzer which attaches via RS232 or USB port to a PC or laptop to give accurate concentration measurements for both ions.細胞一氧化氮測量系統，ArrowSTRAIGHT?細胞一氧化氮測量系統，ArrowSTRAIGHT?細胞一氧化氮測量系統，ArrowSTRAIGHT?細胞一氧化氮測量系統，ArrowSTRAIGHT?