CyToxiLux^® , GranToxiLux^®, and PanToxiLux^?

Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits

A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte

Principle of assays:
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:

CyToxiLux® : downstream caspase

GranToxiLux®: granzyme B

PanToxiLux?: granzyme B and upstream caspase

 

Measurements with CyToxiLux®, GranToxiLux®, and PanToxiLux^TM are superior to bulk assays e.g., LDH and ⁵¹Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).

Detailed CyToxiLux^® PLUS! instructions are available for download in PDF format
Detailed GranToxiLux^® PLUS! instructions are available for download in PDF format
Detailed PanToxiLux^® instructions are available for download in PDF format
Detailed PanToxiLux? P₂D₂ instructions are available for download in PDF format